Milk

Video abstract of our recent paper in Microbiome

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Capturing the diversity of the human milk microbiota through culture-enriched molecular profiling: a feasibility study

Previous human milk studies have confirmed the existence of a highly diverse bacterial community using culture-independent and targeted culture-dependent techniques. However, culture-enriched molecular profiling of milk microbiota has not been done. …

Repeatability and reproducibility assessment in a large-scale population-based microbiota study: case study on human milk microbiota

**Background:** Quality control including assessment of batch variabilities and confirmation of repeatability and reproducibility are integral component of high throughput omics studies including microbiome research. Batch effects can mask true …

Biological observations in microbiota analysis are robust to the choice of 16S rRNA gene sequencing processing algorithm: case study on human milk microbiota

### Background: In recent years, the microbiome field has undergone a shift from clustering-based methods of operational taxonomic unit (OTU) designation based on sequence similarity to denoising algorithms that identify exact amplicon sequence …

Human Milk Bacteria: Seeding the Infant Gut?

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Our milk fungi paper is highlighted by Gut Microbiota For Health

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Breastmilk feeding practices are associated with the co-occurrence of bacteria in mothers’ milk and the infant gut: the CHILD Cohort Study

Gut microbiota play a critical role in infant health. It is now accepted that breastmilk contains live bacteria from endogenous and exogenous sources, but it remains unclear if these bacteria transfer to the infant gut and whether this process is …

Human milk fungi: environmental determinants and inter-kingdom associations with milk bacteria.

**Background:** Fungi constitute an important yet frequently neglected component of the human microbiota with a possible role in health and disease. Fungi and bacteria colonise the infant gastrointestinal tract in parallel, yet most infant microbiome …

Origins of human milk microbiota: new evidence and arising questions

Human milk contains a diverse community of bacteria. The growing appreciation of commensal microbes and increasing availability of high-throughput technology has set the stage for a theory-driven approach to the study of milk microbiota, and …

Integrated Analysis of Human Milk Microbiota with Oligosaccharides and Fatty Acids in the CHILD Cohort

**Background:** Human milk contains many bioactive components that are typically studied in isolation, including bacteria. We performed an integrated analysis of human milk oligosaccharides and fatty acids to explore their associations with milk …